Characterisation, development and validation of UV Spectrophotometric technique for determining Diosmetin in bulk and nanoformulations

Background: From ancient times, India and many other countries have utilised natural products to manage various illnesses. Because they are abundantly available and have fewer adverse effects than synthetic medi-cations, flavonoids are secondary metabolites with diverse biological activities. But till now, no formulations are developed by utilizing Diosmetin and no technique was formulated by utilizing self-biosynthesized Diosmetin (pure). It is a flavone (subclass of flavonoids) isolated from multiple medicinal plants but, in large amounts, is found in citrus plants. They have a variety of biological and pharmacological properties. They also improve lymphatic drainage by raising the duration and severity of lymphatic compression and the exact number of fully operational lymphatic capillaries. Methodology: We have procured Diosmetin from Otto Kemi Pvt. Ltd. and other chemicals from SIGMA chemicals. Furthermore, the characterisation of selected flavone has been performed through solubility studies and method development for development and validation by the UV method for nano-formulation. Result: Diosmetin is pure and soluble in various solvents and has a melting point between 259.14 and 261.84 degrees Celsius, and is more soluble in Acetone, Chloroform, and DMSO, but we have utilised Diosmetin for Cancer studies, so we have taken it in 5% DMSO for further studies. Diosmetin is stable in DMSO till 20 mg/ml at room temperature.