Simultaneous Profiling of Spatial Gene Expression and Chromatin Accessibility During Mouse Brain Development.

Fuqing Jiang,Xin Zhou,Yingying Qian,Miao Zhu,Li Wang,Zhuxia Li,Qingmei Shen, Minhan Wang,Fangfang Qu,Guizhong Cui,Kai Chen,Guangdun Peng

NATURE METHODS（2023）

Cited 7|Views16

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Abstract

The brain is a complex tissue whose function relies on coordinated anatomical and molecular features. However, the molecular annotation of the spatial organization of the brain is currently insufficient. Here, we describe microfluidic indexing-based spatial assay for transposase-accessible chromatin and RNA-sequencing (MISAR-seq), a method for spatially resolved joint profiling of chromatin accessibility and gene expression. By applying MISAR-seq to the developing mouse brain, we study tissue organization and spatiotemporal regulatory logics during mouse brain development.

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Key words

Embryogenesis,Mouse,Neurogenesis,RNA sequencing,Sequencing,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics

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