Correction: Targeted Genetic Changes in Candida albicans Using Transient CRISPR‐Cas9 Expression

Current Protocols is issuing corrections for the following protocol article. Huang, M. Y., Cravener, M. V., & Mitchell, A. P. (2021). Targeted genetic changes in Candida albicans using transient CRISPR-Cas9 expression. Current Protocols, 1, e19. doi: 10.1002/cpz1.19 In the above-referenced article: Some oligo names were reversed and have been corrected throughout the article. In Table 2 the primer name in row 9 has been changed from “YFG1_sgRNA/F” to “YFG1_SNR52/R” and the primer name in row 10 has been changed from “YFG1_sgRNA/R” to “YFG1_sgRNA/F”; On page 4, the primer sequences have been changed as follows: YFG1_sgRNA/F changed from “aaggggttcactgaattctcCAAATTAAAAATAGTTTACGCAAGTC” to “aaggggttcactgaattctcGTTTTAGAGCTAGAAATAGCAAGTTAAA”, and YFG1_SNR52/R: from “gagaattcagtgaaccccttGTTTTAGAGCTAGAAATAGCAAGTTAAA” to “gagaattcagtgaaccccttCAAATTAAAAATAGTTTACGCAAGTC”; In Basic Protocol 3, step 10 has been changed from “Spin down overnight cultures at 1,600 × g for 5 min, discard supernatant, and resuspend cells with 1 ml 15% glycerol in 1:1 dH2O/YPD liquid. Transfer to a 1.5-ml cryo tube and freeze at −80°C for long term storage.” to “Spin down overnight cultures at 1,600 × g for 5 min, discard supernatant, and resuspend cells with 500 µl 30% glycerol and 500 µl YPD liquid. Transfer to a 1.5-ml cryo tube and freeze at −80°C for long term storage.”; In the Upstream Component list on page 15, “pMH01 or pMH06 (50 ng/μl)” has been changed to “pMH02 or pMH05 (50 ng/µl)”; In the annotation note for step 12 on page 18, “This is not recommended due to high volume of colonies.” has been changed to “This is not recommended due to high number of colonies.” In the Reagents and Solutions section, on p18, “Complete synthetic medium without histidine (CMS-His)” has been changed to “Complete synthetic medium without histidine (CSM-His)”. In the Legend to Figure 1, the expected product sizes have been corrected as follows: Ln1: “1015 bp” to “1035 bp” Ln2: “1459 bp” to “1480 bp” Ln3: “∼2474 bp” to “∼2495 bp” Ln7″ “pMH01” changed to “pMH02” and “1966 bp” changed to “2058 bp” Ln8: “pMH02” changed to “pMH01” and “2058 bp” changed to “1966 bp” The current version online now includes these corrections and may be considered the authoritative version of record.