Isolation, culture and, characterization of endometrial mesenchymal stromal cells (endMSCs)

Abstract Endometrial Mesenchymal Stromal Cells (endMSCs) can be isolated from menstrual blood of healthy women and expanded under standard culture conditions. Once the cell culture is established, the multipotency of endMSCs can be in vitro evaluated using specific differentiation media for 21 days. Cell differentiation state can be observed by optical microscopy. On the other hand, the phenotypical characterization can be performed by flow cytometry targeting a combination of surface markers.This protocol is suitable for establishing an endMSCs culture for multiple purposes: in vitro assays, preclinical studies, “omic” analyses, etc.