Combined Treatment Effect of Topical CM-hWJMSCs and Oral Moringa oleifera extract on Wound Healing of Diabetic Rat

AIMS: The aim of the study was to determine the activity of topical conditioned medium (CM) mesenchymal stem cells as single or combined with oral therapy of Moringa oleifera extract (ME) in lowering blood glucose levels and healing diabetic wounds. MATERIALS AND METHODS: Male Sprague-Dawley rats were induced hyperglycemia with Streptozotocin and injured by a biopsy punch on the dorsal side. Then, the rats were divided into five groups, namely, the CM group, which was given topically; the CM–ME group, which was given CM gel topically and ME orally; the CM–Met group, which was given Metformin (Met) as the positive control; commercial gel containing neomycin and placenta extract (BP); and placebo as the negative control. The treatments were given once daily for 16 days. On the 3rd, 8th, and 16th days of treatment, blood glucose level was measured, and anatomical observations of wounds and histology were performed using hematoxylin-eosin and Masson’s Trichrome staining. RESULTS: The wounds treated with CM–ME and CM–Met have a similar profile of lowering blood glucose levels; however, Met was faster to reach blood glucose levels below 250 mg/dL (day 8) compared with ME (day 16). As shown in data on anatomical and histological wound healing parameters, untreated mice exhibited moist wounds with poor healing. Meanwhile, the group that received CM gel alone or along with oral ME showed an improvement in wound parameters. CONCLUSION: CM–ME has a similar profile to CM–Met in reducing blood glucose levels and healing diabetic wounds parameters in rats.