Nanosecond microwave pulses affect stem cell proliferation rate

On 17 cultures of stem cells (SK) isolated from the femur of laboratory rats «Wistar», the change in the rate of cell proliferation in vitro after irradiation with 4000 nanosecond microwave pulses with a pPFD of 140 and 1500 W/cm2 at pulse repetition rates of 8 and 13 Hz was experimentally studied. It has been established that exposure to nanosecond RPMR with both intensities affects the rate of proliferation SK. After exposure to 4000 RPMR pulses with 140 and 1500 W/cm2 pPFD and a pulse repetition rate of 8 Hz, inhibition of cell proliferation was observed relative to the control group. After exposure to a pulse repetition rate of 13 Hz at the same intensities, stimulation of proliferation took place, as a result of which the number of stem cells in the studied cultures increased. The main revealed pattern is the dependence of the effect (on the pulse repetition rate and, in part, on the intensity). Understanding the patterns will allow choosing and using the most optimal mode of exposure to achieve the desired result, and knowledge of the primary mechanism will make it possible to enhance or weaken the effect, in particular, the combined effect of RPMR with other factors.