Potentials of Single Nucleotide Polymorphisms and Genetic Diversity Studies at HSP90AB1 Gene in Nigerian White Fulani, Muturu and N’Dama Cattle Breeds

Abstract The study was aimed at genetic characterization of the (Heat Shock Protein 90AB1) HSP90AB1 gene in the Muturu, N’Dama and White Fulani Nigerian cattle. Total number of 200 blood samples were collected from white Fulani, muturu and N’Dama breeds of cattle out of which 20 samples each were used for the wet laboratory experiment. DNA was extracted from the blood samples using the Zymo-bead extraction kit and DNA sequencing of our samples was performed. A total number of 9 and 11 Single nucleotide polymorphisms (SNPs) were detected using the codon code aligner software within exons 5–6 and 12–13 of the HSP90AB1 gene. ARLEQUIN 2.0001 software was used to estimate the basic population genetic statistics while the DnaSP version5.10.01 was used to estimate the genetic diversity indices. In exons 5–6, the N’Dama cattle had the highest (5) for number of SNPs and genetic diversity indices while other breeds including White Fulani and Muturu had the least (2) number of SNPs and genetic diversity indices breeds. In exons 12–13, White Fulani had the highest (7) for the numbers of SNPs and genetic diversity indices while Muturu had the least (1) number of SNPs and genetic diversity indices. Some of the SNPs identified at these loci were shared by the breeds, suggesting the likelihood of shared ancestral alleles and similar functions. The study concluded that the HSP90AB1 was polymorphic and highly diverse in Nigerian cattle breeds.