Pueraria lobata resistant starch improves obesity by regulating lipid metabolism and gut microbiota in high-fat diet mice

Abstract The health potential of resistant starch (RS) on the gut and its microbiota has received widespread attention. Here, we prepared Pueraria lobata resistant starch (PLRS) by modifying Pueraria lobata starch with Pullulanase and hypothesized that PLRS could improve lipid metabolism disorders and modulate gut microbiota structure in high-fat diet (HFD) induced obese mice. Our results revealed that PLRS alleviated lipid metabolism disorders by ameliorating HFD-induced liver and intestinal damage, significantly upregulating the abundance of Akkermansia, Lactobacillus, Blautia, and Dubosiella, as well as markedly suppressing Staphylococcus in obese mice. Molecular mechanisms have elucidated that the PLRS may exert reducing fat accumulation and stabilize the gut microbiota effects through the modulation of the fatty acid metabolic signaling pathway and the PPAR signaling pathway. Therefore, we suggest that this prebiotic carbohydrate with specific physiological functions could be used to prepare healthy lipid-lowering foods to attenuate obesity problems.