A Nanozyme-linked immunosorbent assay for Aflatoxin B1 detection with High Sensitivity and Excellent Reproducibility

Abstract The nanozyme-linked immunosorbent assay (NLISA) has attracted increasing attention for detecting mycotoxin due to its excellent specificity and low-cost. However, the dispersion of most nanozymes is not desirable, which usually leads to poor reproducibility of detection. Herein, we generated a novel porous Iron-Porphyrin-Zr-MOF NanoPCN-223(Fe) with outstanding dispersion and superior peroxidase-like activity. The excellent dispersion and catalytic performance of NanoPCN-223(Fe) was exploited to establish an enhanced dispersion MOF-linked immunosorbent assay (Ed-MOFLISA) to detect aflatoxin B1 in real food samples. The optimized Ed-MOFLISA displayed a broad quantitative range from 0.05 to 10 ng/mL and a limit of detection of 0.003 ng/mL. The recovery of spiked peanut milk and soy milk ranged from 91.22–97.63%. Intra-assay and inter-assay coefficient of variation values ranged from 0.78 to 3.85%, demonstrating the outstanding reproducibility and accuracy of Ed-MOFLISA. The findings indicate the suitability of novel NanoPCN-223(Fe) with enhanced dispersion for NLISA in food safety analyses.