Guide RNA and pegRNA design and preparation v1

Vikki A. Krysov, Rachel H Wilson, Nicholas S. Ten, Nathan Youlton,Hannah N. De Jong,Shirley Sutton,Yong Huang,Chloe Reuter,Megan E. Grove, Euan A Ashley,Matthew T. Wheeler,Victoria N. Parikh

crossref(2022)

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摘要
This protocol outlines the induction and correction of mutagenesis in HEK293T cells using CRISPR-X and prime editing.
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