Preparation of Cultured Cells for Serial Block Face Scanning Electron Microscopy (SBEM) v1

Daniela Boassa,Thomas J. Deerinck,Eric A. Bushong,Andrea Thor,Mark Ellisman

crossref（2022）

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摘要

Serial block-face scanning electron microscopy (SBEM) is a 3D EM method that allows volume reconstruction of biological samples. This protocol has been adapted for processing of cells in culture grown on glass-bottom dishes.

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