Protocols from: Evolutionary analyses of visual opsin genes in frogs and toads: diversity, duplication, and positive selection v1

Protocols used to extract mRNA from frog retinas, create cDNA libraries, and amplify opsins by PCR for sequencing at the UT core facility under their standard protocols. These protocols were used to obtain the opsin sequences in the paper: Evolutionary analyses of visual opsin genes in frogs and toads: diversity, duplication, and positive selection.