Composite Hemostat Spray Seals Post-Surgical Blood Burst and Ameliorates Bacteria-Arised Inflammation for Expediting Wound Healing

Postoperative bleeding and infection are the main causesof death.Although a commercial zeolite-represented hemostatic agent has beenwidely used, zeolite-induced thermal damages to tissues are unresolved,and antibacterial and wound healing are desirably demanded but challenging.Herein, a composite zeolite-based hemostat spray consisting of Ag-doped beta zeolite (Ag-beta Z) and coupled fibrinogen/thrombin (F/T)has been developed to seal postsurgical blood burst. Systematic experimentsreveal that the F/T can be switched into fibrin gel as the physicalbarrier and cover Ag-beta Z, which imparts the composite hemostatswith the most robust hemostasis ability associated with the lowestbleeding amount and the shortest hemostasis time. Concurrently, thephysical barrier can shield Ag-beta Z from touching water, reducesolvothermal release, and significantly minimize temperature, consequentlyresulting in no obvious thermal effect on the surrounding tissues.Importantly, the in situ doping of Ag can produce reactive oxygenspecies to kill bacteria and ameliorate bacteria-aroused inflammation,which can be also leveraged to promote cell expansion for expeditingwound healing. The clinically approved components guarantee biosafety,paving a solid foundation for clinical translation.