Anti-Psoriasis Inflammation And Anti-Oxidation of The Extract From The Flower of Datura Metel L.

Abstract Background： At present, Flos Datura L. has been reported to have obvious anti-psoriasis effect, but its active ingredient is still unknown. Objectives: The chemical characteristics and anti-psoriasis inflammatory and antioxidant activities of partially purified flavonoids components of Flos Daturae (FC-FD) were investigated.Methods: FC-FD were analyzed by ultra-performance liquid chromatography (UPLC)-electrospray ionization (ESI)-quadrupole time-of-flight (Q-TOF)-mass spectrometry (MSE) combined, while antioxidant potency was monitored using a drosophila model. And the immune inflammatory response was measured in guinea pig animal model.Results: After comparison, 14 products were identified. In vitro antioxidant activity assays showed that FC-FD could effectively scavenge hydroxyl radicals. Furthermore, the longest survival time of the male and female Drosophila melanogaster was extended by 100.50% and 96.82%, respectively. In addition, in the psoriasiform guinea pigs model, FC-FD could decrease the histopathological score and the expression of PCNA protein, and block the production of TNF-α. Notably, the partial purified flavonoids extracted from Flos Datura was and identified for the first time. Conclusions: The results indicated that FC-FD are potential natural antioxidants in extending lifespan and anti-psoriatic inflammatory drugs.