Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay

Maize ear development determines the crop yield,and many important transcription factors(TFs)have been identified to function in this process.However,their transcriptional regulation mechanisms are still elusive.In this study,we generated the genome-wide DNA binding sites for 8 TFs which are known to function or highly expressed in inflorescence by applying the tsCUT&Tag method in maize leaf protoplast.We exposed a regulatory grid of 4 WUSCHEL-related homeobox(WOX)genes and revealed their potential regulatory mechanisms.In addition,a hierarchical regulation network for the determinacy and specifica-tion of maize inflorescence meristems were also constructed using the binding profiles of a floral development gene INDETERMINATE FLORAL APEX1(IFA1)and 3 MADS-box genes.Our study provides an in-depth understanding and new insights of the regulatory mechanisms during maize inflorescence development.