Isolation and Characterization of Staphylococcus Aureus Phage and Its Anti-Biofilm Activity Individually or Collaborative with Streptomycin

Abstract Background: Staphylococcus aureus was a widespread of Gram-positive pathogen bacteria which causes a wide range of symptoms. Bacteria biofilm was the multicellular community of microorganisms that attached to non-biological and biological surfaces. Method: Here, we aimed to isolation and characterization of S. aureus phage and research its bactericidal activity that individually or collaborative with streptomycin.Results: In this study, virulent phage WX was isolated from slaughter house in Jiangsu, China. It’s belonged to the Siphoviridae family and optimal growth temperature was 37 ℃, the pH of optimal preservation buffer was 6~7, optimal multiplicity of infection (MOI) was 0.01 and the genome size was 141, 342 bp. Phage WX has can sterilize most clinical strains of S. aureus which was isolated from clinical patients in the first people's hospital of Yunnan province laboratory. Streptomycin has better anti-biofilm effect than phage WX in low concentration culture of bacteria, nonetheless, phage WX has better anti-biofilm effect than streptomycin in high concentration culture of bacteria. Collaboration of phage WX and streptomycin have better anti-biofilm effect than alone of WX or streptomycin in low concentration culture of bacteria and phage WX have better anti-biofilm effect than streptomycin in high concentration culture of bacteria. Conclusion: The data of this study provided a strong evidence of application phage for reduce the growth of S. aureus biofilm, this study was important for clinic and replace antibiotics in some extent.