LncRNA sequencing analysis of cervical cancer identifies radiotherapy sensitivity markers

Abstract Background Cervical cancer is one of the most common human cancers worldwide. However, the treatment of cervical cancer does not meet patient expectations. In recent years, long noncoding RNAs (lncRNAs), which are nonprotein-coding transcripts, have been shown to play important roles in tumorigenesis and tumor progression. Methods The function and mechanism of lncRNAs in cervical carcinoma are still rarely reported. We selected 15 patients with cervical cancer and a good prognosis (RS) and 15 patients with cervical cancer and a poor prognosis (RR), and we then performed lncRNA Sequencing analysis to detect differentially expressed lncRNAs. Subsequently, we used online bioinformatics databases to explore the potential function of these dysregulated lncRNAs. Finally, quantitative real-time PCR was performed to confirm the expression levels of these dysregulated lncRNAs in sub-RR and RS samples. Results We used lncRNA Sequencing technology to reveal differentially expression between the lncRNA profiles of the RR and RS tissues, and we found 1097 upregulated and 1189 downregulated lncRNAs in cervical cancer (fold change > 2.0), 247 upregulated and 189 downregulated lncRNAs in cervical cancer (fold change > 5.0), and 94 upregulated and 95 downregulated lncRNAs in cervical cancer (fold change > 10.0). Conclusions This study provides a map of lncRNA expression in RR and RS tissues and provides a database for further study of the function and mechanisms of action of key lncRNAs in cervical cancer. LncRNA TCONS_00081487 regulate CD55 and increase the radiation sensitivity of cervical cancer.