Rereferencing of clinical EEGs with nonunipolar mastoid reference to infinity reference by REST.

OBJECTIVE:Conventional electroencephalography (EEG) offline subtraction rereferencing is invalid for many clinical practices when adopting a specific nonunipolar recording montage (e.g., the ipsilateral mastoid (IM) and contralateral mastoid (CM)). Further comparative analyses would thus be blocked due to the lack of a uniform offline reference. Therefore, our goal was to resolve this problem by introducing and assessing the reference electrode standardization technique (REST) to transform nonunipolar mastoid montages into a computational zero reference at infinity (IR) offline. METHODS:For EEG signals and power/connectivity configurations, simulation and clinical schizophrenia resting-state EEG datasets were used to investigate the performance of REST. RESULTS:REST produced small absolute errors (signal level: 1.21-1.26; power: 0.0057-0.021; connectivity: 0.066-0.088) and high correlations (>0.9) between the IM/CM-IR and true IR references. Using clinical data with the IM online reference, REST revealed valuable changes in spectral and connectivity (P < 0.05) in schizophrenia patients, consistent with previous studies. CONCLUSIONS:These results demonstrated that REST transformation could be adopted to resolve the offline rereferencing of clinical EEGs with specific nonunipolar mastoid references. SIGNIFICANCE:REST could be an effective and robust resolution for nonunipolar clinical EEGs and could therefore retrieve these data for further analysis by deriving a favorable offline reference IR.