Sargassum horneri extracts loaded nanostructured lipid carriers: Preparation, physicochemical properties, and in vitro antioxidant activity studies

This study tried to establish optimal conditions for Sargassum horneri extracts loaded nanostructured lipid carriers (SHE-NLCs). Therefore, we observed various physicochemical analyzes, sugar and phenolic contents, and antioxidants while storing the SHE-NLCs for 28 days at different SHE addition amounts (0%–0.5%) and storage temperatures (25 and 65 °C). All SHE-NLCs stored at 25 °C showed a small hydrodynamic diameter (20.1–25.4 nm) and high encapsulation efficiency (64.1%–89.3%) for 28 days. As the amount of SHE increased, the pH and electrical conductivity of SHE-NLCs significantly decreased and increased, respectively (p < 0.05). Also, at 25 °C, prevented a sharp decrease in the total phenolic content and antioxidant activity of SHE-NLCs as the storage period increased compared to 65 °C. SHE 0.25% loaded NLCs can stably maintain the formulation structure for 28 days at room temperature while being an amount sufficiently containing the functionality of SHE.