Structural analysis of the water channel AQP2 by single-particle cryo-EM

Akiko Kamegawa,Shota Suzuki,Hiroshi Suzuki,Kouki Nishikawa,Nobutaka Numoto,Yoshinori Fujiyoshi

Journal of Structural Biology（2023）

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摘要

•The single-particle cryo-EM structure of hAQP2 was solved at 2.9 Å.•A cytoplasmic extension in the density map was likely a highly flexible C-terminus.•Densities corresponding to water molecules were observed in channel pores.•Single-particle cryo-EM can be applied to aquaporins without a fiducial marker.

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关键词

Single-particle cryo-EM, Membrane proteins, Water channel

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