An intelligent and novel electrochemical biosensor for simultaneous enzymatic biosensing of cholesterol and glucose in the presence of uric acid based on first-and second-order calibration methods

•A novel biosensor was fabricated for simultaneous determination of GL and CL.•First- and second-order calibration models were developed.•Second-order calibration using MCR-ALS showed the best performance.•Simultaneous determination of GL and CL was successfully performed.•Performance of the biosensor was comparable with HPLC-UV.