Mapping a leaf rust resistance gene LrOft in durum wheat Ofanto and its suppressor SuLrOft in common wheat.
Frontiers in plant science(2023)
摘要
Epidemics of leaf rust (caused by the fungal pathogen Erikss., ) raise concerns regarding sustainability of wheat production. Deployment of resistant cultivars is the most effective and economic strategy for combating this disease. Ofanto is a durum wheat cultivar that exhibits high resistance to race PHT throughout its entire growing period. In the present study, we identified a leaf rust resistance gene in Ofanto and temporarily designated it as . was mapped to a 2.5 cM genetic interval in chromosome arm 6BL between Indel markers and . During introgression of from Ofanto to common wheat it was observed that F plants of Ofanto crossed with Shi4185 exhibited leaf rust resistance whereas the F of Ofanto crossed with ND4503 was susceptible. In order to map the presumed suppressor locus, a Shi4185/ND4503//Ofanto three-way pentaploid population was generated and was mapped on chromosome arm 2AS. was mapped within a 2.6 cM genetic interval flanked by and . Fine mapping using 2,268 plants of the three-way cross narrowed the suppressor locus to a 68.2-kbp physical interval according to IWGSC RefSeq v1.1. Sequence analysis of genes in the physical interval revealed that encoding an RPP-13-like protein with an NB-ARC domain was a potential candidate for .
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关键词
durum wheat, leaf rust resistance, fine mapping, resistance suppressor, NB-ARC
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