Multi-Probe Nano-Genomic Biosensor to Detect S. aureus from Magnetically-Extracted Food Samples.

One of the most prevalent causes of foodborne illnesses worldwide is staphylococcal food poisoning. This study aimed to provide a robust method to extract the bacteria from food samples using glycan-coated magnetic nanoparticles (MNPs). Then, a cost-effective multi-probe genomic biosensor was designed to detect the nuc gene of rapidly in different food matrices. This biosensor utilized gold nanoparticles and two DNA oligonucleotide probes combined to produce a plasmonic/colorimetric response to inform users if the sample was positive for . In addition, the specificity and sensitivity of the biosensor were determined. For the specificity trials, the biosensor was compared with the extracted DNA of , serovar Enteritidis (SE), and The sensitivity tests showed that the biosensor could detect as low as 2.5 ng/µL of the target DNA with a linear range of up to 20 ng/µL of DNA. With further research, this simple and cost-effective biosensor can rapidly identify foodborne pathogens from large-volume samples.