Detection of Nine Oncogenes Amplification in Lung and Colorectal Cancer Formalin-Fixed Paraffin-Embedded Tissue Samples using Combined Next-Generation Sequencing-Based Script and Digital Droplet Polymerase Chain Reaction.

Christophe Bontoux,Alice Guyard,Audrey Lupo, Karim Diallo,Khedidja Rebah, Agathe Hercent,Edouard Guenzi,Jérome Cros, Jérome Lamoril,Karen Leroy,Nathalie Theou-Anton

Cancer control : journal of the Moffitt Cancer Center（2023）

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摘要

Combined NGS-based script and ddPCR method is reliable and easily feasible for the detection of gene amplifications, providing useful data for guided therapy in cancer.

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关键词

lung cancer, colorectal cancer, sequencing data, copy number, amplification, genes, digital droplet PCR, bioinformatics

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