Elevated atmospheric CO₂ delays the key timing for split N applications to improve wheat (Triticum aestivum L.) protein composition

Late stage nitrogen (N) applications following basic fertilization are commonly used to ensure grain yield and increase grain protein content in wheat. Split N applications at the late growth stage of wheat are an effective measure to improve N absorption and transport and thus increase grain protein content. However, whether split N applications can alleviate the decrease in grain protein content induced by elevated atmospheric CO2 concentrations (e[CO2]) remains unclear. In the present study, a free-air CO2 enrichment system was used to investigate the effects of split N applications (at booting or anthesis) on grain yield, N utilization, protein content, and the composition of wheat under atmospheric (ACO(2); 400 & PLUSMN; 15 ppm) and elevated CO2 concentrations (ECO2; 600 & PLUSMN; 15 ppm). The results showed that wheat grain yield and grain N uptake increased by 5.0% (being grains per ear by 3.0%, 1000-grain weight by 2.0%, and harvest index by 1.6%) and 4.3%, respectively, whereas grain protein content decreased by 2.3% under ECO2 conditions. Although the negative effect of e[CO2] on grain protein content was not alleviated by split N applications, gluten protein content was enhanced due to the alteration of N distribution in different protein fractions (albumins, globulins, gliadins, and glutenins). Compared to that without split N applications, the gluten content of wheat grains increased by 4.2% and 4.5% when late stage N was applied at the booting stage under ACO(2) and anthesis under ECO2 conditions, respectively. The results indicate that rational handling of N fertilizers may be a promising approach to coordinating grain yield and quality under the effects of future climate change. However, compared to ACO(2) conditions, the key timing for improving grain quality by split N applications should be postponed from the booting stage to anthesis under e[CO2] conditions.