Establishment of a convenient ChIP-seq protocol for identification of the histone modification regions in the medicinal plant Andrographis paniculata

Histone modification is essential for gene transcription. In medicinal plants, investigation of the regulation of the secondary metabolites biosynthesis related genes (SMBRGs) has attracted significant attention. However, how histone modifications regulate these important genes is still elusive in medicinal plants, which might be due to the limit of application of high throughput sequencing methods (e.g. ChIP-seq) targeting the histone marks in the genome. Here, we established a convenient ChIP-seq protocol which uses the Tn5 tagmentation library construction strategy to identify genome-wide H3K27me3 enrichment regions in Andrographis paniculata. We believe this protocol will facilitate further investigations on how histone modifications regulate the SMBRGs.