A platform to aid select the optimal tool to design guide rnas

CRISPR-mediated gene-editing technology has arguably driven an unprecedented revolution in biological sciences for its role in elucidating gene functions.A multitude of software has been developed for the design and analysis of CRISPR/Cas experiments,including predictive tools to design optimally guide RNA for various experimental operations.Different in silico sgRNA design tools have various application scenarios and identifying the optimal design tools can often be a challenge.This paper describes the sgRNA design workflow in experiments,the classification of sgRNA designers,previously published benchmarking work of in silico designers,and the criteria involved how to select an sgRNA web server.Through basic testing,this paper comprehensively overviews and compares the features of 43 web server designers to provide a reference for the readers.Ultimately,the project developed an integrated platform,called Aid-TG,which helps users find appropriate tools quickly.