4-Amino 2-Nitrotoluene (4A2NT) Oral Approximate Lethal Dose 14-Day Range Finding 90-Day Subchronic Feeding Studies in Rats, August 1991-November 1993

: The subchronic study examined the toxicity of 4-Amino 2-Nitroluene (4A2NT) in rats following oral administration in feed for 90 days. Associated with the administration of 4A2NT in rats was testicular hypospermatogenesis (atrophy) a higher incidence of cardiomyopathy, anemia and a high incidence of hecatocellular cytoplasmic vacvolization. A no observed adverse effect level in the 90-day study was not established. The lowest-observed-adverse-effect-level in the 90-day study was 27 mg/kg/day for males and 32 mg/kg/day for females.