Identification of gallbladder cancer by direct near-infrared measurement of deuterated chloroform-extracted organic phase from human bile

•A direct NIR measurement of organic phase extracted from human bile was demonstrated.•This scheme was to simply identify gallbladder (GB) cancer against other hepatobiliary diseases.•CDCl3 was used as an extraction medium for solvent interference-free NIR measurement.•The peak intensities of the GB cancer samples were lower than those of the other diseases.•The differences were statistically significant with a confidence interval greater than 99.0%.