Natural occurrence of Beauveria bassiana (Ascomycota: Hypocreales) infecting Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) and earwig in eastern DR Congo

Background The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), poses a threat to the food security of populations in sub-Saharan Africa because of its damage to maize crops. As alternative to the use of hazardous pesticides, microbial control is one of the most promising sustainable approaches adopted to limit the damages caused by S. frugiperda . The sampling targeted mainly larvae of S. frugiperda ; however, during the survey, cadavers of earwig found on the same sampling sites were also collected and involved in the study. Cadavers of targeted insects, with and without sign of fungal infection, were sampled from 3 localities in eastern DR Congo. Culture of fungal isolates was performed in selective Sabouraud dextrose agar media. Results Morphological study of fungal features such as conidia (shape and size) and conidiophores showed that the isolates were from the genus Beauveria . Conidial measurements were highly variable and ranged from 2.4 to 3.6 µm in length and from 1.75 to 3.0 µm in width. Molecular characterization and phylogenetic analysis of the 2 Beauveria isolates based on DNA sequencing of ITS-5.8S region confirmed that both isolates belong to Beauveria bassiana . The 2 isolates of B. bassiana P5E (OP419735.1) and KA14 (OP419734.1) were isolated from cadavers of FAW and earwig, respectively. The alignment with different sequences of B. bassiana from different continent showed that P5E belonged to the same clade of previous isolates reported from Iran and Mexico, while KA14 was with the same clade as isolates from Kenya and China. Conclusion To our knowledge, this is the first study reporting the occurrence of B. bassiana infecting FAW and earwig in eastern DR Congo and in Africa.