谷歌浏览器插件
订阅小程序
在清言上使用

Real‐time Imaging of Nascent DNA in Live Cells by Monitoring the Fluorescence Lifetime of DNA‐Incorporated Thiazole Orange‐Modified Nucleotides

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION(2023)

引用 0|浏览14
暂无评分
摘要
A modified 2′‐deoxycytidine triphosphate derivative ( dC TO TP ) bearing a thiazole orange moiety tethered via an oligoethylene glycol linker was designed and synthesized. The nucleotide was incorporated into DNA by DNA polymerases in vitro as well as in live cells. Upon incorporation of dC TO TP into DNA, the thiazole orange moiety exhibited a fluorescence lifetime that differed significantly from the non‐incorporated (i.e. free and non‐covalently intercalated) forms of dC TO TP . When dC TO TP was delivered into live U‐2 OS cells using a synthetic nucleoside triphosphate transporter, it allowed us to distinguish and monitor cells that were actively synthesizing DNA in real time, from the very first moments after the treatment. We anticipate that this probe could be used to study chromatin organization and dynamics.
更多
查看译文
关键词
DNA nanotechnology,DNA Structure,Triazole Synthesis
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要