LIPID SUPPLEMENTATION IN WARMING MEDIA IMPROVES DEVELOPMENTAL POTENTIAL OF MOUSE OOCYTES AND ZYGOTES

Oocyte and embryo vitrification has been widely used in human assisted reproductive technology (ART) as the preferred approach for fertility preservation. However, vitrified oocytes had compromised developmental potential compared to the fresh oocytes. The objective of this study to examine whether additional of exogenous lipid during warming would improve developmental potential of mouse oocyte and zygotes. We also tested the longevity of the lipid-supplemented media by examining lipid peroxidation and the performance of the media after extended period of storage.