Xonotlite Nanowire-Containing Bioactive Scaffolds for the Therapy of Defective Adipose Tissue in Breast Cancer

Considering the challenge in the treatment of severebreast tumorpatients, xonotlite nanowire-containing bioactive scaffolds (Fe3O4-CS-GelMA) were fabricated by the 3D-printingtechnique for the therapy of injured adipose tissue after surgery.Importantly, benefiting from the excellent magnetothermal performanceof Fe3O4 microspheres, Fe3O4-CS-GelMA scaffolds could effectively kill tumor cells invitro and suppress breast cancer in vivo under an alternating magnetic field, and the tumor did not recurin 2 weeks. In addition, attributed to the released bioactive inorganicions, Fe3O4-CS-GelMA composite scaffolds couldeffectively promote the expression of adipogenesis-related genes andproteins of adipose-derived stem cells (ADSCs) via the PI3K-AKT signalingpathway in vitro. Furthermore, Fe3O4-CS-GelMA scaffolds with ADSCs could obviously stimulate theformation of adipose in vivo, compared with thatof pure GelMA without inorganic components. Therefore, this studyoffers a promising strategy for the therapy of breast tumors afterthe surgical excision of breast carcinoma.