Cannabidiol Analogue CIAC001 for the Treatment of Morphine-Induced Addiction by Targeting PKM2.

Opioid addiction is a chronically relapsing disorder that causes critical public health problems. Currently, there is a lack of effective drug treatment. Herein, one cannabidiol derivative, , was discovered as an effective agent for treating morphine-induced addiction. In vitro, exhibited significantly improved anti-neuroinflammatory activity with lower toxicity. In vivo, ameliorated the morphine-induced withdrawal reaction, behavioral sensitization, and conditional position preference by inhibiting morphine-induced microglia activation and neuroinflammation. Target fishing for by activity-based protein profiling led to the identification of pyruvate kinase M2 (PKM2) as the target protein. bound to the protein-protein interface of the PKM2 dimer and promoted the tetramerization of PKM2. Moreover, exhibited an anti-neuroinflammatory effect by reversing the decrease of the PKM2 tetramer and inhibiting the nuclear translocation of PKM2. In summary, this study identified as a lead compound in targeting PKM2 to treat morphine-induced addiction.