Analytical Strategies and Method Validation to Access the Bioavailability of Essential Elements and Arsenic from Raw and Cooked Fish and Shrimp
ACS FOOD SCIENCE & TECHNOLOGY(2023)
摘要
The bioavailability of essential and toxic elements inraw/cookedtilapia and shrimp was estimated using in vitro gastrointestinaldigestion followed by the Caco-2 cell model to simulate the humanintestinal barrier. Analytical strategies such as aerosol dilution,collision cell, and matrix-matching calibration were successfullyexplored for multielement quantification using ICP OES and ICP-MS.From Caco-2 cell bioassays, the estimated bioavailability values rangedfrom 1.4% (Fe) to 15.2% (Cu) in tilapia and from 0.2% (Fe) to 10.7%(P) in shrimp. Air-frying and oven-baking methods decreased tilapia'sMg, P, S, Cu, and Fe bioavailability. Contrarily, the bioavailabilityof essential elements (except Mn) in shrimp seemed to increase afterair-frying. Arsenic bioavailability in cooked shrimp was significantlyhigher than in the raw sample (1.9 vs 5.7%) but fortunatelybelow the limit allowed by legislation. In spite of the complexityof basal solutions from the cell-based bioassays, the applied analyticalstrategies allowed the accurate quantification of the bioavailablefractions by ICP-based methods.
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关键词
thermal treatments,Caco-2 cells,invitro uptake,analytical strategies
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