[Chloroplast genomic characterization and phylogenetic analysis of Pellionia scabra ].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology(2023)

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摘要
belongs to the genus in the family of , and is a high-quality wild vegetables with high nutritional value. In this study, high-throughput techniques were used to sequence, assemble and annotate the chloroplast genome. We also analyzed its structure, and construct the phylogenetic trees from the . to further study the chloroplast genome characteristics. The results showed that the chloroplast genome size was 153 220 bp, and the GC content was 36.4%, which belonged to the typical tetrad structure in . . The chloroplast genome encodes 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes in . . Among them, 15 genes contained 1 intron, 2 genes contained 2 introns, and had trans-splicing, respectively. In . , chloroplast genomes could be divided into four categories, including 43 photosynthesis, 64 self-replication, other 7 coding proteins, and 4 unknown functions. A total of 51 073 codons were detected in the chloroplast genome, among which the codon encoding leucine (Leu) accounted for the largest proportion, and the codon preferred to use A and U bases. There were 72 simple sequence repeats (SSRs) in the chloroplast genome of . , containing 58 single nucleotides, 12 dinucleotides, 1 trinucleotide, and 1 tetranucleotide. The 1 gene expansion was present at the IRb/SSC boundary. The phylogenetic trees showed that . (OL800583) was most closely related to (MZ292972), (MK227819) and var. (MN189961). Taken together, our results provide worthwhile information for understanding the identification, genetic evolution, and genomics research of . species.
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chloroplast genomic characterization,pellionia scabra,phylogenetic analysis
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