Withdrawal: Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts

Hypoxia-inducible Factor 1 (HIF-1) Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in FibroblastsJournal of Biological ChemistryVol. 288Issue 15PreviewBackground: In hypoxic cells, HIF-1 transactivates genes with roles in developmental, physiological, and disease processes.Results: HIF-1 activity in hypoxic fibroblasts promotes extracellular matrix (ECM) remodeling by inducing expression of the collagen hydroxylases P4HA1, P4HA2, and PLOD2.Conclusion: HIF-1 stimulates ECM fiber alignment, which influences cell morphology, adhesion, and directional migration.Significance: Hypoxia induces changes in ECM that have non-cell-autonomous effects on cell behavior. Full-Text PDF Open Access This article has been withdrawn by the authors, except S. Bajpai and P. Chaturvedi who could not be reached. The authors and journal concluded figure 1 presented background issues on ‘FN’ panel, lane 1. In figure 2B, ‘FN’ panel corresponding to ‘Conditioned media’ (bottom panel in the middle), lanes 1 and 4 were reused. In addition, the top right panel for ‘ECM’, lanes 3 and 4 were reused. Finally, ‘Conditioned media’ top middle panel, lanes 1 and 4 were reused. Figure 3B ‘HIF 1a’ top panel, lanes 5 and 7 were reused. In supplemental figure S1 A, in ‘HIF1’ panel lanes 2 and 4 were reused. In supplemental figure S1 B, ‘HIF2a’ panel, lanes 1 and 3 were reused. In addition, ‘Actin’ panel lanes 3 and 4 were spliced in. In supplemental figure S1 B, ‘P4HA1’ panel lane 1 was spliced in, and lanes 4 and 6 were reused. In supplemental figure S1 B, ‘P4HA2’ panel, lanes 4 and 5 were reused. In supplemental figure S1 C, ‘HIF1a’ panel, lanes 3 and 4 were reused. In supplemental figure S1 D, ‘P4HA1’ panel on top left, lanes 1 and 2 are spliced together with lanes 3 and 4. In supplemental figure S1 D, ‘P4HA2’ top middle panel, lane 1 was spliced in. The original data provided did not resolve these issues.