Editorial: The protagonism of bioanalytical methods in high-throughput drug discovery

Two original research manuscripts, one review, and one mini-review on analytical assays for ligand screening are collected in this Research Topic, covering assays for ornithine decarboxylase inhibitor screening, on-flow enzymatic inhibitor screening through liquid chromatography methods, liquid chromatography coupled to mass spectrometry (LC-MS) method to screen human kallikrein (KLKs) inhibitors, and a study on salt concentration to improve the separation performance of biomarkers for transporter protein inhibition. In the following paragraphs, each published manuscript is presented and briefly described.The ornithine decarboxylase (ODC) enzyme belongs to the polyamine biosynthetic pathway, catalyzing the decarboxylation of ornithine to putrescine. Polyamines (putrescine, spermidine, and spermine) are essential growth factors in eukaryotic cells, but their high levels are associated with carcinogenesis (Gerner and Meyskens, 2004) and Alzheimer's disease (Mäkitie et al., 2010