Noise Investigation in Femtosecond Stimulated Raman Scattering Microscopy.

Stimulated Raman Scattering (SRS) microscopy enables imaging systems, performing label-free imaging with high sensitivity, high spatial and spectral resolution, 3D sectioning, and fast image acquisition. Nevertheless, due to the weak Raman cross-section of biomolecules, SRS images often suffer from low SNR, so SRS applications in biological/biomedical imaging can be compromised. In this paper, noise measurements in detection system of our in-house built Stimulated Raman Scattering microscope are carried out, demonstrating that it is shot noise limited.