Micro-Fabricated Optics for Multiphoton Microscopy
ICTON(2023)
摘要
We report new methods of 2 photon polymerization of microlenses with high numerical aperture, large diameter and good optical quality. We characterize the aberrations of these lenses that, coupled to raster scanning optical microscopes, allow two-photon excitation imaging of cells. In-vivo non-linear imaging will be also discussed, opening the possibility to use these micro-lenses in implants for the continuous inspection of biological dynamics in vivo.
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关键词
2-photon polymerization,micro-lenses,optical microscopy,non-linear excitation microscopy,label free imaging
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