Novosphingobium beihaiensis sp. nov., a novel pesticide-tolerant bacterium isolated from mangrove sediments

A novel Novosphingobium species, designated strain B2638 T , was isolated from mangrove sediments which was collected from Beibu Gulf, Beihai, P. R. China. The isolate could grow in the presence of chlorpyrifos. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the isolate belonged to the genus Novosphingobium , showing 99.9% sequence similarity with N. decloroationis 502str22 T and less than 98% similarity with other type strain of species of this genus. Molecular typing by BOX-PCR divided strain B2638 T and N. declorationis 502str22 T into two clusters and indicated that they were not identical. Genomic comparison referenced by values of the DNA-DNA hybridization (dDDH) and the average nucleotide identity (ANI) between strain B2638 T and its close phylogenetic neighbors were 20.0–29.5% and 75.3–85.3%, respectively, that were lower than proposed thresholds for bacterial species delineation. The major fatty acids (> 10%) were identified as C 18:1 ω 7c, C 17:1 iso ω 9c and C 16:0 . The main polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, phosphatidyl glycerol, unidentified lipid and unidentified aminolipid. Results from phenotypic, chemotaxonomic and genotypic analyses proposed that strain B2638 T (= MCCC 1K07406 T = KCTC 72968 T ) is represented a novel species in the genus Novosphingobium , for which the names Novosphingobium beihaiensis sp. nov. is proposed.