Evaluation of Cuscuta reflexa seed essential oil on TPA-induced inflammation in mice

Objectives: Cuscuta reflexa is parasitic plant of subtropical and temperate areas, commonly named as amarbel or akasvel in India. Present study is to investigate the In-vivoanti-inflammatory potential of C. reflexa seed essential oil. Materials and methods: In this study the essential oil was hydro-distilled from dried seeds and analyzed for chemical profiling and anti-inflammatory activity. In -vivo activity was performed on mice inducing TPA as disease causing agent.Study of biochemical parameter and oxidative stress was performed by taking homogenate of ear pinna of mice. Results: Chemical analysis is done by GC and GC-MS study, we found b-Bisabolene and Hexahydro farnesyl acetone as the major component as 13.2 and 14.2% respectively along with cis-chrysanthenyl acetate, caryophyllene oxide, carotol, caryophyllene oxide. In-vitro activity of cell viability was assessed using RAW 264.7 macrophages, showed no reduction in viable cell in the processed culture. In-vivo it significantly reduces the inflammation by inhibiting pro-inflammatory cytokines (IL-1b, IL-6 and TNF-a). Conclusions: Cuscuta reflexa seed essential oil was found effective in the management of inflammation by encountering the inflammatory mediators (cytokines). & COPY; 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).