Droplet based low input proteomic platform for rare cell populations
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS, an accessible low-input platform that generates high-fidelity proteomic profiles of 100 - 2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, discovering and validating CD36 as a marker of progenitor capacity in the basal cell compartment. We anticipate DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.
### Competing Interest Statement
The authors have declared no competing interest.
更多查看译文
关键词
proteomic platform,droplet,cell,low input
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要