Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation.

Andrew Wesley Legan,Hillary L Mehl, Alex Varaksa,Kenneth A Callicott

Microbiology resource announcements（2023）

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摘要

Toxic molds in the genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.

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关键词

Aspergillus, transcription, aflatoxin, annotation, Nanopore

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