Cryo-EM structures reveal native GABAA receptor assemblies and pharmacology

NATURE(2023)

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摘要
Type A gamma-aminobutyric acid receptors (GABA(A)Rs) are the principal inhibitory receptors in the brain and the target of a wide range of clinical agents, including anaesthetics, sedatives, hypnotics and antidepressants(1-3). However, our understanding of GABA(A)R pharmacology has been hindered by the vast number of pentameric assemblies that can be derived from 19 different subunits(4) and the lack of structural knowledge of clinically relevant receptors. Here, we isolate native murine GABA(A)R assemblies containing the widely expressed alpha 1 subunit and elucidate their structures in complex with drugs used to treat insomnia (zolpidem (ZOL) and flurazepam) and postpartum depression (the neurosteroid allopregnanolone (APG)). Using cryo-electron microscopy (cryo-EM) analysis and single-molecule photobleaching experiments, we uncover three major structural populations in the brain: the canonical alpha 1 beta 2 gamma 2 receptor containing two alpha 1 subunits, and two assemblies containing one alpha 1 and either an alpha 2 or alpha 3 subunit, in which the single alpha 1-containing receptors feature a more compact arrangement between the transmembrane and extracellular domains. Interestingly, APG is bound at the transmembrane alpha/beta subunit interface, even when not added to the sample, revealing an important role for endogenous neurosteroids in modulating native GABA(A)Rs. Together with structurally engaged lipids, neurosteroids produce global conformational changes throughout the receptor that modify the ion channel pore and the binding sites for GABA and insomnia medications. Our data reveal the major alpha 1-containing GABA(A)R assemblies, bound with endogenous neurosteroid, thus defining a structural landscape from which subtype-specific drugs can be developed. Using cryo-EM, structures of three major assemblies of type A GABA receptors, which regulate brain excitability, are revealed in the mouse brain and provide a basis for the development of subtype-specific drugs.
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native gabaa receptor assemblies
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