Analytical and preparative chiral supercritical fluid chromatography resolutions using crown ether-derived column

In this study, crown ether-derived column Crownpak & REG; CR-I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide-based columns. Crownpak & REG; CR-I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak & REG; CR-I (+). The first reported preparative SFC separations on Crownpak & REG; CR-I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstrate that Crownpak & REG; CR-I (+) is a very useful column in the chiral separation of challenging compounds that contain a primary amine group in the pharmaceutical industry. Crown ether-derived chiral stationary phase (CSP) offers superior resolution over polysaccharide-based CSP for the SFC resolution of primary amines and can be used at both analytical and preparative scales.image