Long Amplification PCR (LA-PCR) Detection of Azole Resistant Trichophyton indotineae

Trichphyton indotineae , a species newly designated in 2020 independent of T. interdigitale , comprises highly terbinafine (TRF)-resistant dermatophytosis that is epidemic in North India and spreding to worldwide. Some clinical isolates of T. indotineae have been resistance both TRF and azoles that might be caused the treatment failure. To detect the azole resistance strains, we developed a long amplification PCR (LA-PCR) detection method for the tandem repeat of the CYP51B (encoding sterol 14a-demethylase gene) in T. indotineae . Contrasting the drug susceptibility test results with the LA-PCR results confirmed a trend toward low susceptibility to azole antifungal agents in strains with amplifications of 9.5 kbp or greater (3 or more copies of CYP51B ). Our results suggest that the method could be detected rapidly of low-susceptibility strains to azole antifungal agents.