New Perylene-Based Chemiluminescent Polymer Nanoparticles for Highly Selective Detection of the Superoxide Anion In Vivo.
Analytical chemistry(2023)
摘要
The superoxide anion (O) is one of the primary reactive oxygen species in biological systems. Developing a determination system for O in vivo has attracted much attention thanks to its complex biological function. Herein, we proposed a new perylene-based chemiluminescence (CL) probe, the SH-PDI polymer, which was capable of generating strong CL signals with O in comparison with other ROS. The CL mechanism involved was proposed to be a kind of oxidation reaction induced by the breakage of the S-S and S-H bonds into sulfoxide bonds by O. Subsequently, a nanoprecipitation method was introduced, using cumene-terminated poly(styrene--maleic anhydride) as the amphiphilic agent, to obtain water-soluble nanoparticles, SPPS NPs, which exhibited not only stronger CL intensity but also higher selectivity toward O than the SH-PDI polymer. Moreover, the CL wavelength of the SPPS-O system was found to be located at 580 and 710 nm, which was conducive to CL imaging. By virtue of these advantages, SPPS NPs were utilized to evaluate the O level in vitro in the range of 0.25-60 μM at pH 7.0, with a detection limit of 8.2 × 10 M (S/N = 3). Moreover, SPPS NPs were also capable of imaging O in an LPS-induced acute inflammation mice model and drug-induced acute kidney injury (AKI).
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关键词
chemiluminescent polymer nanoparticles,superoxide anion,perylene-based
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