FIGURE 5 from Metastatic Competency and Tumor Spheroid Formation Are Independent Cell States Governed by RB in Lung Adenocarcinoma

RB restoration reverses metastatic competency. A, Contingency analysis of the number of mice injected with single-cell suspensions produced from Group 1 tumor spheroids and treated with tamoxifen (RbR/R) or vehicle (RbTR/TR). Outcome of interest is tumor formation 5 weeks after injection. Statistical significance was assessed by χ2 test. For KP;RbTR/TR mice, n = 9, and for KP;RbR/R mice, n = 8. B, Number of tumors forming in mice five weeks after injection with single-cell suspensions produced from Group 1 tumor spheroids. Statistical significance assessed by Student t test. For KP;RbTR/TR mice, n = 9, and for KP;RbR/R mice, n = 8. Error bars represent mean ± SD. C, Lung mass in mice 5 weeks after injection with single-cell suspensions produced from Group 1 tumor spheroids. Statistical significance assessed by Student t test. For KP;RbTR/TR mice, n = 9, and for KP;RbR/R mice, n = 8. Error bars represent mean ± SD. D, Representative lung sections stained with hematoxylin and eosin from mice injected with single-cell suspensions produced from Group 1 tumor spheroids. Lung section images were taken with a Leica DMI6000 using a 5X objective.