Mir-153 Targets Autophagy and Apoptosis Caspase Family to Explore the Molecular Mechanism of Polystyrene Nanoparticles Intervening in Thoracoabdominal Aortic Aneurysm

This study investigates whether miR-153 regulates the expression of autophagy and apoptosis-related gene Caspase3, and analyzes the role of polystyrene nanoparticles in thoracoabdominal aortic aneurysm cells. Surgically resected thoracoabdominal aortic aneurysm tissue samples were included as the observation group and the control group included 2 cm tissues from the side of thoracoabdominal aortic aneurysm. The mRNA levels of miR-153 and Caspase3 were assessed by reverse transcription polymerase chain reaction (RT-PCR) and cell proliferation, apoptosis, invasion, and migration were evaluated. Tissue microarray and RT-PCR showed that miR-153 was expressed in tissues and it displayed a targeted relationship with Caspase3. Transwell reslts showd that transfection of miR-153-targeted Caspase3 P: 2038 10920 On: Tue 05 Sep 2023 06:29:43 nanoparticles significantly reduced the infiltration of thoraciand aortic anerysm. After si-RNA interfered with the expresCopyright: American Scientific Publishers sion of miR-153, the proliferation rate of thoracoabdominalDeliveredaor bytic anurngentaysms in the miR-153-targeted Caspase3-directed nanoparticle group was significantly reduced. On the contrary, when miR-153 was overexpressed, the apoptosis was significantly reduced. Transfection of si-miR-153-targeting Caspase3 nanoparticles significantly reduced the invasion and metastasis ability of thoracoabdominal aortic aneurysm cells and the migration ability was increased after reversing miR153 expression. The decreased expression of miR-153 is related to the increased expression of Caspase3 and enhanced invasion and metastasis of thoracoabdominal aortic aneurysms. Overexpression of miR-153 can inhibit the expression of Caspase3 and targeted nanoparticles can effectively enhance cell activities.